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After incubation, 2 μl of 5 × First-strand RT buffer, 0.5 μl of 0.1 M dithiothreitol (DTT), 0.5 μl of RNaseOUT™ (40 U/μl), and 0.5 μl SuperScript III Reverse Transcriptase (200 U/μl) were added and incubated at 25 °C for 10 min, and then 50 °C for 50 min, followed by the final termination at 85 °C for 5 min