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Real-time quantitative polymerase chain reaction (RT-qPCR) The samples were added into Trizol for cracking, then chloroform was added for centrifugation to obtain the upper liquid phase, and isopropyl alcohol was added for centrifugation to obtain the precipitate

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These data indicate that d-allulose ameliorates basal hyperglycemia and impaired glucose tolerance (IGT) in HFD-fed mice via GLP-1R signaling