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In the 1980s, researchers at the NIH were studying something that seemed purely curiosity-driven: venom from the Gila monster, a desert lizard. What they found changed medicine. A molecule in that venom —

Step 3 Batch Process Description: To a nanofiltered DMF solution of Preparation 29 (2.249 g, 46.5 mg/g, 104.6 bmg, 0.0244 mmol) and Preparation 30 (71.5 mg, 90.6 area%, 0.0306 mmol) in DMF (0.3068 g, 0.325 mL) at -5°C is added a 5.0 wt% DIEA solution in DMF (114.0 mg, 5.70 mg DIEA, 0.0441 mmol, 1.8 equivalents) and a 10.1 wt% solution of (l-[bis(dimethylamino)methylene]-lH-l,2,3-triazolo[4,5-b]pyridinium 3- oxide hexafluorophosphate (HATU) in DMF (154.9 mg, 15.59 mg HATU, 0.0410 mmol, 1.7 equivalents)

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Real‐Time Quantitative Reverse Transcription PCR was used to quantify genes by SYBR Green (Bio‐Rad), and relative abundance of each mRNA was normalized to beta‐actin mRNA